Publications

Bao XX, Spanos C, Kojidani T, Lynch EM, Rappsilber J, Hiraoka Y, Haraguchi T, Sawin KE. Exportin Crm1 is repurposed as a docking protein to generate microtubule organizing centers at the nuclear pore. Elife. 2018 May 29. doi: 10.7554/eLife.33465. PMID: 29809148

Carpy A, Koch A, Bicho CC, Borek WE, Hauf S, Sawin KE, Maček B. Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technology in Fission Yeast. Cold Spring Harb Protoc. 2017 Jun 1. doi: 10.1101/pdb.top079814.. PMID: 28572211

Carpy A, Koch A, Bicho CC, Borek WE, Hauf S, Sawin KE, Maček B. Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Quantitative Proteomics and Phosphoproteomics in Fission Yeast. Cold Spring Harb Protoc. 2017 Jun 1. doi: 10.1101/pdb.prot091686. PMID: 28572185

Koch A, Bicho CC, Borek WE, Carpy A, Maček B, Hauf S, Sawin KE. Construction, Growth, and Harvesting of Fission Yeast Stable Isotope Labeling by Amino Acids Cell Culture (SILAC) Strains. Cold Spring Harb Protoc. 2017 Jun 1. doi: 10.1101/pdb.prot091678. PMID: 28572184

Mutavchiev DR, Leda M, Sawin KE. Remodeling of the Fission Yeast Cdc42 Cell-Polarity Module via the Sty1 p38 Stress-Activated Protein Kinase Pathway. Current Biology 2016. doi: 10.1016/j.cub.2016.08.048.PMID: 27746023

Borek WE, Groocock LM, Samejima I, Zou J, de Lima Alves F, Rappsilber J, Sawin KE. Mto2 multisite phosphorylation inactivates non-spindle microtubule nucleation complexes during mitosis.Nat Commun. 2015 Aug 5;6:7929. doi: 10.1038/ncomms8929. PMID: 26243668

Borek WE, Zou J, Rappsilber J, Sawin KE. Deletion of Genes Encoding Arginase Improves Use of "Heavy" Isotope-Labeled Arginine for Mass Spectrometry in Fission Yeast. PLoS One. 2015 Jun 15;10(6):e0129548. doi: 10.1371/journal.pone.0129548. eCollection 2015. PMID: 26075619

Rincon SA, Bhatia P, Bicho C, Guzman-Vendrell M, Fraisier V, Borek WE, Alves Fde L, Dingli F, Loew D, Rappsilber J, Sawin KE, Martin SG, Paoletti A. Pom1 regulates the assembly of Cdr2-Mid1 cortical nodes for robust spatial control of cytokinesis. J Cell Biol. 2014 Jul 7;206(1):61-77. doi: 10.1083/jcb.201311097. Epub 2014 Jun 30.

Lynch EM, Groocock LM, Borek W, Sawin KE.Activation of the g-Tubulin Complex by the Mto1/2 Complex, Current Biology 2014.doi: 10.1016/j.cub.2014.03.006J.

Snaith HA, Thompson J, Yates JR 3rd, Sawin KE. Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis. J Cell Sci. 2011 Jul 1;124(Pt 13):2187-99. doi: 10.1242/jcs.084038. Epub 2011 Jun 7. PubMed PMID: 21652630; PubMed Central PMCID: PMC3113670.

Anders A, Sawin KE. Microtubule stabilization in vivo by nucleation-incompetent γ-tubulin complex. J Cell Sci. 2011 Apr 15;124(Pt 8):1207-13. doi: 10.1242/jcs.083741. PubMed PMID: 21444751; PubMed Central PMCID: MC3065382.

Samejima I, Miller VJ, Rincon SA, Sawin KE. Fission yeast Mto1 regulates diversity of cytoplasmic microtubule organizing centers. Curr Biol. 2010 Nov 9;20(21):1959-65. doi: 10.1016/j.cub.2010.10.006. Epub 2010 Oct 21.PubMed PMID: 20970338; PubMed Central PMCID: PMC2989437.

Bicho CC, Kelly DA, Snaith HA, Goryachev AB, Sawin KE. A catalytic role for Mod5 in the formation of the Tea1 cell polarity landmark. Curr Biol. 2010 Oct 12;20(19):1752-7. doi: 10.1016/j.cub.2010.08.035. Epub 2010 Sep 16. PubMed PMID: 20850323; PubMed Central PMCID: PMC3094757.

Snaith HA, Anders A, Samejima I, Sawin KE. New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol. 2010;97:147-72. doi: 10.1016/S0091-679X(10)97009-X. Review. PubMed PMID: 20719270.

Bicho CC, de Lima Alves F, Chen ZA, Rappsilber J, Sawin KE. A genetic engineering solution to the "arginine conversion problem" in stable isotope labeling by amino acids in cell culture (SILAC). Mol Cell Proteomics. 2010 Jul;9(7):1567-77. doi: 10.1074/mcp.M110.000208. Epub 2010 May 10. PubMed PMID: 20460254; PubMed Central PMCID: PMC2896365.

Sawin KE, Bicho CC, Snaith HA. Inexpensive synthetic-based matrix for both conventional and rapid purification of protein A- and tandem affinity purification-tagged proteins. Anal Biochem. 2010 Feb 15;397(2):241-3. doi: 10.1016/j.ab.2009.09.045. Epub 2009 Sep 30. PubMed PMID: 19799851; PubMed Central PMCID: PMC2810510.

Sawin KE. Cell cycle: Cell division brought down to size. Nature. 2009 Jun 11;459(7248):782-3. doi: 10.1038/459782a. PubMed PMID: 19516326.

Anders A, Watt S, Bähler J, Sawin KE. Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1. Yeast. 2008 Dec;25(12):913-25. doi: 10.1002/yea.1639. PubMed PMID: 19160458; PubMed Central PMCID: PMC2964509.

Samejima I, Miller VJ, Groocock LM, Sawin KE. Two distinct regions of Mto1 are required for normal microtubule nucleation and efficient association with the gamma-tubulin complex in vivo. J Cell Sci. 2008 Dec 1;121(Pt 23):3971-80. doi: 10.1242/jcs.038414. Epub 2008 Nov 11. PubMed PMID: 19001497; PubMed Central PMCID: PMC2743986.

Taylor SS, Hardwick KG, Sawin KE, Biggins S, Piatti S, Khodjakov A, Rieder CL, Salmon ED, Musacchio A. Comment on "A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint". Science. 2007 May 18;316(5827):982; author reply 982. PubMed PMID: 17510347; PubMed Central PMCID:PMC2590763.

Sawin KE. Cell division: mid-level management. Curr Biol. 2007 Feb 6;17(3):R93-5. PubMed PMID: 17276908.

Sawin KE, Tran PT. Cytoplasmic microtubule organization in fission yeast. Yeast. 2006 Oct 15;23(13):1001-14. Review. PubMed PMID: 17072892.

Anders A, Lourenço PC, Sawin KE. Noncore components of the fission yeast gamma-tubulin complex. Mol Biol Cell. 2006 Dec;17(12):5075-93. Epub 2006 Oct 4. PubMed PMID: 17021256; PubMed Central PMCID: PMC1679674.

Snaith HA, Samejima I, Sawin KE. Multistep and multimode cortical anchoring of tea1p at cell tips in fission yeast. EMBO J. 2005 Nov 2;24(21):3690-9. Epub 2005 Oct 13. PubMed PMID: 16222337; PubMed Central PMCID: PMC1276721.

Sawin KE. Meiosis: organizing microtubule organizers. Curr Biol. 2005 Aug 23;15(16):R633-5. Review. PubMed PMID: 16111936.

Snaith HA, Sawin KE. Tea for three: control of fission yeast polarity. Nat Cell Biol. 2005 May;7(5):450-1. PubMed PMID: 15867927.

Samejima I, Lourenço PC, Snaith HA, Sawin KE. Fission yeast mto2p regulates microtubule nucleation by the centrosomin-related protein mto1p. Mol Biol Cell. 2005 Jun;16(6):3040-51. Epub 2005 Jan 19. PubMed PMID: 15659644; PubMed Central PMCID: PMC1142446.

Sawin KE. Microtubule dynamics: faint speckle, hidden dragon. Curr Biol. 2004 Sep 7;14(17):R702-4. Review. PubMed PMID: 15341759.

Sawin KE, Lourenco PC, Snaith HA. Microtubule nucleation at non-spindle pole body microtubule-organizing centers requires fission yeast centrosomin-related protein mod20p. Curr Biol. 2004 May 4;14(9):763-75. PubMed PMID: 15120067.

Sawin KE. The spindle gets bigger. Cell. 2004 Apr 30;117(3):281-3. PubMed PMID: 15109489.

Sawin KE, Snaith HA. Role of microtubules and tea1p in estalishment and maintenance of fission yeast cell polarity. J Cell Sci. 2004 Feb 15;117(Pt 5):689-700. Epub 2004 Jan 20. PubMed PMID: 14734657

Snaith HA, Sawin KE. Fission yeast mod5p regulates polarized growth through anchoring of tea1p at cell tips. Nature. 2003 Jun 5;423(6940):647-51. PubMed PMID: 12789340.

Sawin KE. Cell polarity: following formin function. Curr Biol. 2002 Jan 8;12(1):R6-8. PubMed PMID: 11790314.

Sawin KE. Microtubule dynamics: the view from the tip. Curr Biol. 2000 Nov 30;10(23):R860-2. PubMed PMID: 11114532.

Sawin KE. Cytokinesis: Sid signals septation. Curr Biol. 2000 Jul 27-Aug 10;10(15):R547-50. Review. PubMed PMID: 10959826.

Sawin KE, Hajibagheri MA, Nurse P. Mis-specification of cortical identity in a fission yeast PAK mutant. Curr Biol. 1999 Nov 18;9(22):1335-8. PubMed PMID:10574765.

Sawin KE. Some thoughts about microtubules and cell polarity in fission yeast. Fungal Genet Biol. 1999 Jul-Aug;27(2-3):224-30. Review. PubMed PMID: 10441448.

Sawin KE. GFP fusion proteins as probes for cytology in fission yeast. Methods Cell Biol. 1999;58:123-38. Review. PubMed PMID: 9891378.

Sawin KE, Nurse P. Regulation of cell polarity by microtubules in fission yeast. J Cell Biol. 1998 Jul 27;142(2):457-71. PubMed PMID: 9679144; PubMed Central PMCID: PMC2133047.

Mitchison TJ, Sawin KE, Theriot JA, Gee K, Mallavarapu A. Caged fluorescent probes. Methods Enzymol. 1998;291:63-78. PubMed PMID: 9661145.

Sawin KE, Nurse P. Photoactivation of green fluorescent protein. Curr Biol. 1997 Oct 1;7(10):R606-7. PubMed PMID: 9368737.

Sawin KE, Nurse P. Identification of fission yeast nuclear markers using random polypeptide fusions with green fluorescent protein. Proc Natl Acad Sci USA. 1996 Dec 24;93(26):15146-51. PubMed PMID: 8986778; PubMed Central PMCID: PMC26371.

Sawin KE, Mitchison TJ. Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle. Proc Natl Acad Sci USA. 1995 May 9;92(10):4289-93. PubMed PMID: 7753799; PubMed Central PMCID: PMC41929.

Fox LA, Sawin KE, Sale WS. Kinesin-related proteins in eukaryotic flagella. J Cell Sci. 1994 Jun;107 ( Pt 6):1545-50. PubMed PMID: 7962196.

Sawin KE, Mitchison TJ. Microtubule flux in mitosis is independent of chromosomes, centrosomes, and antiparallel microtubules. Mol Biol Cell. 1994 Feb;5(2):217-26. PubMed PMID: 8019007; PubMed Central PMCID: PMC301027.

Hogan CJ, Wein H, Wordeman L, Scholey JM, Sawin KE, Cande WZ. Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain. Proc Natl Acad Sci USA. 1993 Jul 15;90(14):6611-5. PubMed PMID: 8341676; PubMed Central PMCID: PMC46982.

Sawin KE, Endow SA. Meiosis, mitosis and microtubule motors. Bioessays. 1993 Jun;15(6):399-407. Review. PubMed PMID: 8357342.

Sawin KE, LeGuellec K, Philippe M, Mitchison TJ. Mitotic spindle organization by a plus-end-directed microtubule motor. Nature. 1992 Oct 8;359(6395):540-3. PubMed PMID: 1406972.

Sawin KE, Mitchison TJ, Wordeman LG. Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies. J Cell Sci. 1992 Feb;101 ( Pt 2):303-13. PubMed PMID: 1629247.

Sawin KE, Scholey JM. Motor proteins in cell division. Trends Cell Biol. 1991 Nov;1(5):122-9. PubMed PMID: 14731543.

Sawin KE, Mitchison TJ. Poleward microtubule flux mitotic spindles assembled in vitro. J Cell Biol. 1991 Mar;112(5):941-54. PubMed PMID: 1999464; PubMed Central PMCID: PMC2288886.

Sawin KE, Mitchison TJ. Mitotic spindle assembly by two different pathways in vitro. J Cell Biol. 1991 Mar;112(5):925-40. PubMed PMID: 1999463; PubMed Central PMCID: PMC2288871.

Belmont LD, Hyman AA, Sawin KE, Mitchison TJ. Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts. Cell. 1990 Aug 10;62(3):579-89. PubMed PMID: 2379239.

Mitchison TJ, Sawin KE. Tubulin flux in the mitotic spindle: where does it come from, where is it going? Cell Motil Cytoskeleton. 1990;16(2):93-8. Review. PubMed PMID: 2198113.