Publications

Mutavchiev DR, Leda M, Sawin KE. Remodeling of the Fission Yeast Cdc42 Cell-Polarity Module via the Sty1 p38 Stress-Activated Protein Kinase Pathway. Current Biology 2016. doi: 10.1016/j.cub.2016.08.048.PMID: 27746023

Borek WE, Groocock LM, Samejima I, Zou J, de Lima Alves F, Rappsilber J, Sawin KE. Mto2 multisite phosphorylation inactivates non-spindle microtubule nucleation complexes during mitosis.Nat Commun. 2015 Aug 5;6:7929. doi: 10.1038/ncomms8929. PMID: 26243668

Borek WE, Zou J, Rappsilber J, Sawin KE. Deletion of Genes Encoding Arginase Improves Use of "Heavy" Isotope-Labeled Arginine for Mass Spectrometry in Fission Yeast. PLoS One. 2015 Jun 15;10(6):e0129548. doi: 10.1371/journal.pone.0129548. eCollection 2015. PMID: 26075619

Rincon SA, Bhatia P, Bicho C, Guzman-Vendrell M, Fraisier V, Borek WE, Alves Fde L, Dingli F, Loew D, Rappsilber J, Sawin KE, Martin SG, Paoletti A. Pom1 regulates the assembly of Cdr2-Mid1 cortical nodes for robust spatial control of cytokinesis. J Cell Biol. 2014 Jul 7;206(1):61-77. doi: 10.1083/jcb.201311097. Epub 2014 Jun 30.

Lynch EM, Groocock LM, Borek W, Sawin KE.Activation of the g-Tubulin Complex by the Mto1/2 Complex, Current Biology 2014.doi: 10.1016/j.cub.2014.03.006J.

Snaith HA, Thompson J, Yates JR 3rd, Sawin KE. Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis. J Cell Sci. 2011 Jul 1;124(Pt 13):2187-99. doi: 10.1242/jcs.084038. Epub 2011 Jun 7. PubMed PMID: 21652630; PubMed Central PMCID: PMC3113670.

Anders A, Sawin KE. Microtubule stabilization in vivo by nucleation-incompetent γ-tubulin complex. J Cell Sci. 2011 Apr 15;124(Pt 8):1207-13. doi: 10.1242/jcs.083741. PubMed PMID: 21444751; PubMed Central PMCID: MC3065382.

Samejima I, Miller VJ, Rincon SA, Sawin KE. Fission yeast Mto1 regulates diversity of cytoplasmic microtubule organizing centers. Curr Biol. 2010 Nov 9;20(21):1959-65. doi: 10.1016/j.cub.2010.10.006. Epub 2010 Oct 21.PubMed PMID: 20970338; PubMed Central PMCID: PMC2989437.

Bicho CC, Kelly DA, Snaith HA, Goryachev AB, Sawin KE. A catalytic role for Mod5 in the formation of the Tea1 cell polarity landmark. Curr Biol. 2010 Oct 12;20(19):1752-7. doi: 10.1016/j.cub.2010.08.035. Epub 2010 Sep 16. PubMed PMID: 20850323; PubMed Central PMCID: PMC3094757.

Snaith HA, Anders A, Samejima I, Sawin KE. New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation. Methods Cell Biol. 2010;97:147-72. doi: 10.1016/S0091-679X(10)97009-X. Review. PubMed PMID: 20719270.

Bicho CC, de Lima Alves F, Chen ZA, Rappsilber J, Sawin KE. A genetic engineering solution to the "arginine conversion problem" in stable isotope labeling by amino acids in cell culture (SILAC). Mol Cell Proteomics. 2010 Jul;9(7):1567-77. doi: 10.1074/mcp.M110.000208. Epub 2010 May 10. PubMed PMID: 20460254; PubMed Central PMCID: PMC2896365.

Sawin KE, Bicho CC, Snaith HA. Inexpensive synthetic-based matrix for both conventional and rapid purification of protein A- and tandem affinity purification-tagged proteins. Anal Biochem. 2010 Feb 15;397(2):241-3. doi: 10.1016/j.ab.2009.09.045. Epub 2009 Sep 30. PubMed PMID: 19799851; PubMed Central PMCID: PMC2810510.

Sawin KE. Cell cycle: Cell division brought down to size. Nature. 2009 Jun 11;459(7248):782-3. doi: 10.1038/459782a. PubMed PMID: 19516326.

Anders A, Watt S, Bähler J, Sawin KE. Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1. Yeast. 2008 Dec;25(12):913-25. doi: 10.1002/yea.1639. PubMed PMID: 19160458; PubMed Central PMCID: PMC2964509.

Samejima I, Miller VJ, Groocock LM, Sawin KE. Two distinct regions of Mto1 are required for normal microtubule nucleation and efficient association with the gamma-tubulin complex in vivo. J Cell Sci. 2008 Dec 1;121(Pt 23):3971-80. doi: 10.1242/jcs.038414. Epub 2008 Nov 11. PubMed PMID: 19001497; PubMed Central PMCID: PMC2743986.

Taylor SS, Hardwick KG, Sawin KE, Biggins S, Piatti S, Khodjakov A, Rieder CL, Salmon ED, Musacchio A. Comment on "A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint". Science. 2007 May 18;316(5827):982; author reply 982. PubMed PMID: 17510347; PubMed Central PMCID:PMC2590763.

Sawin KE. Cell division: mid-level management. Curr Biol. 2007 Feb 6;17(3):R93-5. PubMed PMID: 17276908.

Sawin KE, Tran PT. Cytoplasmic microtubule organization in fission yeast. Yeast. 2006 Oct 15;23(13):1001-14. Review. PubMed PMID: 17072892.

Anders A, Lourenço PC, Sawin KE. Noncore components of the fission yeast gamma-tubulin complex. Mol Biol Cell. 2006 Dec;17(12):5075-93. Epub 2006 Oct 4. PubMed PMID: 17021256; PubMed Central PMCID: PMC1679674.

Snaith HA, Samejima I, Sawin KE. Multistep and multimode cortical anchoring of tea1p at cell tips in fission yeast. EMBO J. 2005 Nov 2;24(21):3690-9. Epub 2005 Oct 13. PubMed PMID: 16222337; PubMed Central PMCID: PMC1276721.

Sawin KE. Meiosis: organizing microtubule organizers. Curr Biol. 2005 Aug 23;15(16):R633-5. Review. PubMed PMID: 16111936.

Snaith HA, Sawin KE. Tea for three: control of fission yeast polarity. Nat Cell Biol. 2005 May;7(5):450-1. PubMed PMID: 15867927.

Samejima I, Lourenço PC, Snaith HA, Sawin KE. Fission yeast mto2p regulates microtubule nucleation by the centrosomin-related protein mto1p. Mol Biol Cell. 2005 Jun;16(6):3040-51. Epub 2005 Jan 19. PubMed PMID: 15659644; PubMed Central PMCID: PMC1142446.

Sawin KE. Microtubule dynamics: faint speckle, hidden dragon. Curr Biol. 2004 Sep 7;14(17):R702-4. Review. PubMed PMID: 15341759.

Sawin KE, Lourenco PC, Snaith HA. Microtubule nucleation at non-spindle pole body microtubule-organizing centers requires fission yeast centrosomin-related protein mod20p. Curr Biol. 2004 May 4;14(9):763-75. PubMed PMID: 15120067.

Sawin KE. The spindle gets bigger. Cell. 2004 Apr 30;117(3):281-3. PubMed PMID: 15109489.

Sawin KE, Snaith HA. Role of microtubules and tea1p in estalishment and maintenance of fission yeast cell polarity. J Cell Sci. 2004 Feb 15;117(Pt 5):689-700. Epub 2004 Jan 20. PubMed PMID: 14734657

Snaith HA, Sawin KE. Fission yeast mod5p regulates polarized growth through anchoring of tea1p at cell tips. Nature. 2003 Jun 5;423(6940):647-51. PubMed PMID: 12789340.

Sawin KE. Cell polarity: following formin function. Curr Biol. 2002 Jan 8;12(1):R6-8. PubMed PMID: 11790314.

Sawin KE. Microtubule dynamics: the view from the tip. Curr Biol. 2000 Nov 30;10(23):R860-2. PubMed PMID: 11114532.

Sawin KE. Cytokinesis: Sid signals septation. Curr Biol. 2000 Jul 27-Aug 10;10(15):R547-50. Review. PubMed PMID: 10959826.

Sawin KE, Hajibagheri MA, Nurse P. Mis-specification of cortical identity in a fission yeast PAK mutant. Curr Biol. 1999 Nov 18;9(22):1335-8. PubMed PMID:10574765.

Sawin KE. Some thoughts about microtubules and cell polarity in fission yeast. Fungal Genet Biol. 1999 Jul-Aug;27(2-3):224-30. Review. PubMed PMID: 10441448.

Sawin KE. GFP fusion proteins as probes for cytology in fission yeast. Methods Cell Biol. 1999;58:123-38. Review. PubMed PMID: 9891378.

Sawin KE, Nurse P. Regulation of cell polarity by microtubules in fission yeast. J Cell Biol. 1998 Jul 27;142(2):457-71. PubMed PMID: 9679144; PubMed Central PMCID: PMC2133047.

Mitchison TJ, Sawin KE, Theriot JA, Gee K, Mallavarapu A. Caged fluorescent probes. Methods Enzymol. 1998;291:63-78. PubMed PMID: 9661145.

Sawin KE, Nurse P. Photoactivation of green fluorescent protein. Curr Biol. 1997 Oct 1;7(10):R606-7. PubMed PMID: 9368737.

Sawin KE, Nurse P. Identification of fission yeast nuclear markers using random polypeptide fusions with green fluorescent protein. Proc Natl Acad Sci USA. 1996 Dec 24;93(26):15146-51. PubMed PMID: 8986778; PubMed Central PMCID: PMC26371.

Sawin KE, Mitchison TJ. Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle. Proc Natl Acad Sci USA. 1995 May 9;92(10):4289-93. PubMed PMID: 7753799; PubMed Central PMCID: PMC41929.

Fox LA, Sawin KE, Sale WS. Kinesin-related proteins in eukaryotic flagella. J Cell Sci. 1994 Jun;107 ( Pt 6):1545-50. PubMed PMID: 7962196.

Sawin KE, Mitchison TJ. Microtubule flux in mitosis is independent of chromosomes, centrosomes, and antiparallel microtubules. Mol Biol Cell. 1994 Feb;5(2):217-26. PubMed PMID: 8019007; PubMed Central PMCID: PMC301027.

Hogan CJ, Wein H, Wordeman L, Scholey JM, Sawin KE, Cande WZ. Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain. Proc Natl Acad Sci USA. 1993 Jul 15;90(14):6611-5. PubMed PMID: 8341676; PubMed Central PMCID: PMC46982.

Sawin KE, Endow SA. Meiosis, mitosis and microtubule motors. Bioessays. 1993 Jun;15(6):399-407. Review. PubMed PMID: 8357342.

Sawin KE, LeGuellec K, Philippe M, Mitchison TJ. Mitotic spindle organization by a plus-end-directed microtubule motor. Nature. 1992 Oct 8;359(6395):540-3. PubMed PMID: 1406972.

Sawin KE, Mitchison TJ, Wordeman LG. Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies. J Cell Sci. 1992 Feb;101 ( Pt 2):303-13. PubMed PMID: 1629247.

Sawin KE, Scholey JM. Motor proteins in cell division. Trends Cell Biol. 1991 Nov;1(5):122-9. PubMed PMID: 14731543.

Sawin KE, Mitchison TJ. Poleward microtubule flux mitotic spindles assembled in vitro. J Cell Biol. 1991 Mar;112(5):941-54. PubMed PMID: 1999464; PubMed Central PMCID: PMC2288886.

Sawin KE, Mitchison TJ. Mitotic spindle assembly by two different pathways in vitro. J Cell Biol. 1991 Mar;112(5):925-40. PubMed PMID: 1999463; PubMed Central PMCID: PMC2288871.

Belmont LD, Hyman AA, Sawin KE, Mitchison TJ. Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts. Cell. 1990 Aug 10;62(3):579-89. PubMed PMID: 2379239.

Mitchison TJ, Sawin KE. Tubulin flux in the mitotic spindle: where does it come from, where is it going? Cell Motil Cytoskeleton. 1990;16(2):93-8. Review. PubMed PMID: 2198113.