Publications | Sawin Lab

Microtubule-independent movement of the fission yeast nucleus.

Sanju Ashraf, Ye Dee Tay, David A Kelly, Kenneth E Sawin

Journal of cell science · 26 Mar 2021

Identification of 15 New Bypassable Essential Genes of Fission Yeast.

Aoi Takeda, Shigeaki Saitoh, Hiroyuki Ohkura, Kenneth E Sawin, Gohta Goshima

Cell structure and function · 31 Aug 2019

Architecture of the Mto1/2 microtubule nucleation complex

Harish C. Thakur, Eric M. Lynch, Weronika E. Borek, Xun X. Bao, Sanju Ashraf, Juan Zou, Juri Rappsilber, Atlanta G. Cook, Kenneth E. Sawin

bioRxiv · 31 Aug 2019

Reconstitution of Microtubule Nucleation In Vitro Reveals Novel Roles for Mzt1.

Su Ling Leong, Eric M Lynch, Juan Zou, Ye Dee Tay, Weronika E Borek, Maarten W Tuijtel, Juri Rappsilber, Kenneth E Sawin

Current biology : CB · 08 Jul 2019

Fission Yeast NDR/LATS Kinase Orb6 Regulates Exocytosis via Phosphorylation of the Exocyst Complex.

Ye Dee Tay, Marcin Leda, Christos Spanos, Juri Rappsilber, Andrew B Goryachev, Kenneth E Sawin

Cell reports · 05 Feb 2019

Local and global Cdc42 guanine nucleotide exchange factors for fission yeast cell polarity are coordinated by microtubules and the Tea1-Tea4-Pom1 axis.

Ye Dee Tay, Marcin Leda, Andrew B Goryachev, Kenneth E Sawin

Journal of cell science · 19 Jul 2018

Exportin Crm1 is repurposed as a docking protein to generate microtubule organizing centers at the nuclear pore.

Xun X Bao, Christos Spanos, Tomoko Kojidani, Eric M Lynch, Juri Rappsilber, Yasushi Hiraoka, Tokuko Haraguchi, Kenneth E Sawin

eLife · 29 May 2018

Construction, Growth, and Harvesting of Fission Yeast Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Strains.

André Koch, Claudia C Bicho, Weronika E Borek, Alejandro Carpy, Boris Maček, Silke Hauf, Kenneth E Sawin

Cold Spring Harbor protocols · 01 Jun 2017

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)-Based Quantitative Proteomics and Phosphoproteomics in Fission Yeast.

Alejandro Carpy, André Koch, Claudia C Bicho, Weronika E Borek, Silke Hauf, Kenneth E Sawin, Boris Maček

Cold Spring Harbor protocols · 01 Jun 2017

Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) Technology in Fission Yeast.

Boris Maček, Alejandro Carpy, André Koch, Claudia C Bicho, Weronika E Borek, Silke Hauf, Kenneth E Sawin

Cold Spring Harbor protocols · 01 Jun 2017

Remodeling of the Fission Yeast Cdc42 Cell-Polarity Module via the Sty1 p38 Stress-Activated Protein Kinase Pathway.

Delyan R Mutavchiev, Marcin Leda, Kenneth E Sawin

Current biology : CB · 13 Oct 2016

Mto2 multisite phosphorylation inactivates non-spindle microtubule nucleation complexes during mitosis.

Weronika E Borek, Lynda M Groocock, Itaru Samejima, Juan Zou, Flavia de Lima Alves, Juri Rappsilber, Kenneth E Sawin

Nature communications · 05 Aug 2015

Deletion of Genes Encoding Arginase Improves Use of "Heavy" Isotope-Labeled Arginine for Mass Spectrometry in Fission Yeast.

Weronika E Borek, Juan Zou, Juri Rappsilber, Kenneth E Sawin

PloS one · 15 Jun 2015

Pom1 regulates the assembly of Cdr2–Mid1 cortical nodes for robust spatial control of cytokinesis

Sergio A. Rincon, Payal Bhatia, Claudia Bicho, Mercè Guzman-Vendrell, Vincent Fraisier, Weronika E. Borek, Flavia de Lima Alves, Florent Dingli, Damarys Loew, Juri Rappsilber, Kenneth E. Sawin, Sophie G. Martin, Anne Paoletti

Journal of Cell Biology · 30 Jun 2014

Activation of the γ-tubulin complex by the Mto1/2 complex.

Eric M Lynch, Lynda M Groocock, Weronika E Borek, Kenneth E Sawin

Current biology : CB · 03 Apr 2014

Characterization of Mug33 reveals complementary roles for actin cable-dependent transport and exocyst regulators in fission yeast exocytosis

Hilary A Snaith, James Thompson, John R Yates, Kenneth E Sawin

Journal of cell science · 01 Jul 2011

Microtubule stabilization in vivo by nucleation-incompetent γ-tubulin complex

Andreas Anders, Kenneth E Sawin

Journal of cell science · 15 Apr 2011

Fission yeast Mto1 regulates diversity of cytoplasmic microtubule organizing centers

Itaru Samejima, Victoria J Miller, Sergio A Rincon, Kenneth E Sawin

Current biology : CB · 09 Nov 2010

A catalytic role for Mod5 in the formation of the Tea1 cell polarity landmark

Claudia C Bicho, David A Kelly, Hilary A Snaith, Andrew B Goryachev, Kenneth E Sawin

Current biology : CB · 12 Oct 2010

A genetic engineering solution to the "arginine conversion problem" in stable isotope labeling by amino acids in cell culture (SILAC)

Claudia C Bicho, Flavia de Lima Alves, Zhuo A Chen, Juri Rappsilber, Kenneth E Sawin

Molecular & cellular proteomics : MCP · 01 Jul 2010

Inexpensive synthetic-based matrix for both conventional and rapid purification of protein A- and tandem affinity purification-tagged proteins

Kenneth E Sawin, Claudia C Bicho, Hilary A Snaith

Analytical biochemistry · 15 Feb 2010

New and old reagents for fluorescent protein tagging of microtubules in fission yeast; experimental and critical evaluation.

Hilary A Snaith, Andreas Anders, Itaru Samejima, Kenneth E Sawin

Methods in cell biology · 01 Jan 2010

Cell cycle: Cell division brought down to size.

Kenneth E Sawin

Nature · 11 Jun 2009

Two distinct regions of Mto1 are required for normal microtubule nucleation and efficient association with the gamma-tubulin complex in vivo

Itaru Samejima, Victoria J Miller, Lynda M Groocock, Kenneth E Sawin

Journal of cell science · 01 Dec 2008

Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1

Andreas Anders, Stephen Watt, Jürg Bähler, Kenneth E Sawin

Yeast (Chichester, England) · 01 Dec 2008

Improved tools for efficient mapping of fission yeast genes: identification of microtubule nucleation modifier mod22-1 as an allele of chromatin- remodelling factor gene swr1.

Andreas Anders, Stephen Watt, Jürg Bähler, Kenneth E Sawin

Yeast (Chichester, England) · 01 Dec 2008

Comment on "A centrosome-independent role for gamma-TuRC proteins in the spindle assembly checkpoint"

Stephen S Taylor, Kevin G Hardwick, Kenneth E Sawin, Sue Biggins, Simonetta Piatti, Alexey Khodjakov, Conly L Rieder, Edward D Salmon, Andrea Musacchio

Science (New York, N.Y.) · 18 May 2007

Cell division: mid-level management.

Kenneth E Sawin

Current biology : CB · 06 Feb 2007

Noncore components of the fission yeast gamma-tubulin complex

Andreas Anders, Paula C C Lourenço, Kenneth E Sawin

Molecular biology of the cell · 01 Dec 2006

Cytoplasmic microtubule organization in fission yeast.

Kenneth E Sawin, P T Tran

Yeast (Chichester, England) · 15 Oct 2006

Multistep and multimode cortical anchoring of tea1p at cell tips in fission yeast

Hilary A Snaith, Itaru Samejima, Kenneth E Sawin

The EMBO journal · 02 Nov 2005

Meiosis: organizing microtubule organizers.

Kenneth E Sawin

Current biology : CB · 23 Aug 2005

Fission yeast mto2p regulates microtubule nucleation by the centrosomin-related protein mto1p

Itaru Samejima, Paula C C Lourenço, Hilary A Snaith, Kenneth E Sawin

Molecular biology of the cell · 01 Jun 2005

Tea for three: control of fission yeast polarity.

Hilary A Snaith, Kenneth E Sawin

Nature cell biology · 01 May 2005

Microtubule dynamics: faint speckle, hidden dragon.

Kenneth E Sawin

Current biology : CB · 07 Sep 2004

Microtubule nucleation at non-spindle pole body microtubule-organizing centers requires fission yeast centrosomin-related protein mod20p.

Kenneth E Sawin, Paula C C Lourenco, Hilary A Snaith

Current biology : CB · 04 May 2004

The spindle gets bigger.

Kenneth E Sawin

Cell · 30 Apr 2004

Role of microtubules and tea1p in establishment and maintenance of fission yeast cell polarity.

Kenneth E Sawin, Hilary A Snaith

Journal of cell science · 20 Jan 2004

Fission yeast mod5p regulates polarized growth through anchoring of tea1p at cell tips.

Hilary A Snaith, Kenneth E Sawin

Nature · 05 Jun 2003

Cell polarity: following formin function.

Kenneth E Sawin

Current biology : CB · 08 Jan 2002

Microtubule dynamics: the view from the tip.

K E Sawin

Current biology : CB · 30 Nov 2000

Cytokinesis: Sid signals septation

Kenneth E Sawin

Current Biology · 01 Aug 2000

Mis-specification of cortical identity in a fission yeast PAK mutant.

K E Sawin, M A Hajibagheri, P Nurse

Current biology : CB · 18 Nov 1999

Some Thoughts about Microtubules and Cell Polarity in Fission Yeast

Kenneth E. Sawin

Fungal Genetics and Biology · 01 Jul 1999

GFP fusion proteins as probes for cytology in fission yeast.

K E Sawin

Methods in cell biology · 01 Jan 1999

Regulation of cell polarity by microtubules in fission yeast

K E Sawin, P Nurse

The Journal of cell biology · 27 Jul 1998

Caged fluorescent probes.

T J Mitchison, K E Sawin, J A Theriot, K Gee, A Mallavarapu

Methods in enzymology · 01 Jan 1998

Photoactivation of green fluorescent protein.

K E Sawin, P Nurse

Current biology : CB · 01 Oct 1997

Identification of fission yeast nuclear markers using random polypeptide fusions with green fluorescent protein

K E Sawin, P Nurse

Proceedings of the National Academy of Sciences of the United States of America · 24 Dec 1996

Mutations in the kinesin-like protein Eg5 disrupting localization to the mitotic spindle

K E Sawin, T J Mitchison

Proceedings of the National Academy of Sciences of the United States of America · 09 May 1995

L A Fox, K E Sawin, W S Sale

Journal of cell science · 01 Jun 1994

Microtubule flux in mitosis is independent of chromosomes, centrosomes, and antiparallel microtubules

K E Sawin, T J Mitchison

Molecular biology of the cell · 01 Feb 1994

Inhibition of anaphase spindle elongation in vitro by a peptide antibody that recognizes kinesin motor domain

C J Hogan, H Wein, L Wordeman, J M Scholey, K E Sawin, W Z Cande

Proceedings of the National Academy of Sciences of the United States of America · 15 Jul 1993

Meiosis, mitosis and microtubule motors.

K E Sawin, S A Endow

BioEssays : news and reviews in molecular, cellular and developmental biology · 01 Jun 1993

Mitotic spindle organization by a plus-end-directed microtubule motor.

K E Sawin, K LeGuellec, M Philippe, T J Mitchison

Nature · 08 Oct 1992

Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies.

K E Sawin, T J Mitchison, L G Wordeman

Journal of cell science · 01 Feb 1992

Motor proteins in cell division.

K E Sawin, J M Scholey

Trends in cell biology · 01 Nov 1991

Mitotic spindle assembly by two different pathways in vitro

K E Sawin, T J Mitchison

The Journal of cell biology · 01 Mar 1991

Poleward microtubule flux mitotic spindles assembled in vitro

K E Sawin, T J Mitchison

The Journal of cell biology · 01 Mar 1991

Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts.

L D Belmont, A A Hyman, K E Sawin, T J Mitchison

Cell · 10 Aug 1990

Tubulin flux in the mitotic spindle: where does it come from, where is it going?

T J Mitchison, K E Sawin

Cell motility and the cytoskeleton · 01 Jan 1990